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Promega empty pgl3-basic plasmid
Empty Pgl3 Basic Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+pgl3+basic+plasmids/pgl3+basic/pm36403704-86-19-42
Average 90 stars, based on 1 article reviews
empty pgl3-basic plasmid - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Nuclear factor interleukin 3 (NFIL3) participates in regulation of the NF-κB-mediated inflammation and antioxidant system in Litopenaeus vannamei under ammonia-N stress.
Article Snippet: Nuclear factor interleukin 3 (NFIL3) is a critical upstream regulator of the NF-κB pathway.. Nevertheless, the detailed molecular mechanism of NFIL3 and its function in shrimp have not been well characterized.. In the present study, NFIL3 was identified and characterized from Litopenaeus vannamei.

Article Title: Transcription factor RUNX1 regulates platelet PCTP (Phosphatidylcholine transfer protein): Implications for cardiovascular events Differential Effects of RUNX1 variants
Article Snippet: Cells (2×10 5 ) were co-transfected with 200 ng of PCTP promoter plasmids and either the empty PGL3-basic plasmid (Promega, Madison, WI) or PGL3-basic plasmid containing the PCTP promoter region and 10 ng of pRL-TK vector DNA (Promega) as an internal control transfection.

Article Title: Functional haplotypes of ARID4A affect promoter activity and semen quality of bulls.
Article Snippet: The AT-rich interaction domain 4 A (ARID4A) has an important role in regulating Sertoli cell function and male fertility.. Its molecular mechanisms, however, remain largely unknown.. In this study, two single nucleotide polymorphisms (SNPs) (g.53 G > T, ss 1966531596, and g.826 G > A, rs 210809648) were identified in the promoter region of ARID4A in 215 Chinese Holstein bulls using polymerase chain reaction (PCR)-restriction fragment length polymorphism and created restriction site-PCR.

Article Title: Lysine acetyltransferase 14 mediates TGF-β-induced fibrosis in ovarian endometrioma via co-operation with serum response factor.
Article Snippet: For the luciferase assay, the pretreated primary human EcESCs were plated on 24-well plates and co-transfected with 0.5 μg of pGL3-α-SMA-pro-Luc or empty (pGL3-Basic) plasmids together with 0.05 mg of pRL Renilla luciferase reporter vector (#E2241, Promega, RRID: Addgene_44379) using Lipofectamine 3000 according to the manufacturer’s instructions.

Article Title: Functional analysis of polymorphism haplotypes of MGMT in residents of high background radiation area.
Article Snippet: To investigate the distribution of polymorphisms and their frequent haplotypes in the regulatory region of MGMT in residents of high background radiation area (HBRA) and their impacts on transcriptional activity, we collected DNA samples from 83 healthy Chinese residents in HBRA and searched for genetic polymorphisms in the regulatory region of MGMT.. Haplotypes were characterized by Haploview analysis.. Transcriptional activities of different polymorphism haplotypes were detected by using a dual-luciferase reporter assay.

Article Title: PLCz Functional Haplotypes Modulating Promoter Transcriptional Activity Are Associated with Semen Quality Traits in Chinese Holstein Bulls
Article Snippet: Cells were cotransfected with 50 ng of the pRL-TK vector DNA (Promega) and 400 ng of either the empty pGL3-Basic plasmid (a promoterless control from Promega) or one of the promoter constructs with different lengths and haplotypes of the PLCz promoter.

Article Title: Functional Analysis of Promoter Variants in Genes Involved in Sex Steroid Action, DNA Repair and Cell Cycle Control
Article Snippet: Similar experiments were performed with the empty promoterless pGL3-Basic plasmid (Promega) and the SV40-driven Firefly Luciferase pGL3-control plasmid (Promega, Madison, WI, USA) as negative and positive controls, respectively.

Cotransfection:

Article Title: Regulation of Cytochrome P450 2a5 by Artemisia capillaris and 6,7-Dimethylesculetin in Mouse Hepatocytes
Article Snippet: As a positive control, hepatocytes were co-transfected with pGL3 control plasmid (Promega, Madison, WI, United States) (500 ng/well) which constitutively expresses the luciferase gene. .. Co-transfection with the empty pGL3 basic plasmid (Promega, Madison, WI, United States) (500 ng/well) served as a negative control. ..

Plasmid Preparation:

Article Title: Regulation of Cytochrome P450 2a5 by Artemisia capillaris and 6,7-Dimethylesculetin in Mouse Hepatocytes
Article Snippet: As a positive control, hepatocytes were co-transfected with pGL3 control plasmid (Promega, Madison, WI, United States) (500 ng/well) which constitutively expresses the luciferase gene. .. Co-transfection with the empty pGL3 basic plasmid (Promega, Madison, WI, United States) (500 ng/well) served as a negative control. ..

Negative Control:

Article Title: Regulation of Cytochrome P450 2a5 by Artemisia capillaris and 6,7-Dimethylesculetin in Mouse Hepatocytes
Article Snippet: As a positive control, hepatocytes were co-transfected with pGL3 control plasmid (Promega, Madison, WI, United States) (500 ng/well) which constitutively expresses the luciferase gene. .. Co-transfection with the empty pGL3 basic plasmid (Promega, Madison, WI, United States) (500 ng/well) served as a negative control. ..



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Results from gene reporter assays and transcriptional activity of the characterized promotor haplotypes.
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Results from gene reporter assays and transcriptional activity of the characterized promotor haplotypes.

Journal: Genes

Article Title: Functional Analysis of Promoter Variants in Genes Involved in Sex Steroid Action, DNA Repair and Cell Cycle Control

doi: 10.3390/genes10030186

Figure Lengend Snippet: Results from gene reporter assays and transcriptional activity of the characterized promotor haplotypes.

Article Snippet: Similar experiments were performed with the empty promoterless pGL3-Basic plasmid (Promega) and the SV40-driven Firefly Luciferase pGL3-control plasmid (Promega, Madison, WI, USA) as negative and positive controls, respectively.

Techniques: Activity Assay, Luciferase, Plasmid Preparation, Clone Assay, Control, Expressing